Semaglutide Reconstitution Guide

Discover how to safely reconstitute Semaglutide.

Reconstitution Steps

Start with clean hands to ensure a sterile environment.

Assemble all necessary items including the semaglutide vial, bacteriostatic water vial, syringe with needle, alcohol wipe.

Determine the desired concentration of the solution for accurate dosing.

Add bacteriostatic water carefully to achieve the correct reconstitution.

Gently swirl the vial to dissolve the peptide, avoiding shaking.

Allow the powder to fully dissolve. If undissolved particles remain, use a sterile syringe filter to remove any particles.

Concentration of Semaglutide

To prepare the required concentration of semaglutide, you must calculate the volume of solvent (bacteriostatic water) to add to the semaglutide vial. Use the following formula to determine this:

Here are examples of how to use this formula for specific concentrations in 2 mg and 5 mg vials:

Example 1: Reconstituting a 2 mg vial for a 1 mg/mL concentration:

To achieve a 1 mg/mL concentration using 2 mg of powdered medication, apply the formula as follows. Thus, you would need to add 2 mL of bacteriostatic water to the vial with 2 mg of powder to reach a concentration of 1 mg/mL.

Example 2: Reconstituting a 5 mg vial for a 2.4 mg/mL concentration:

For a concentration of 2.4 mg/mL with 5 mg of powdered medication, the calculation is:

Therefore, you should add approximately 2.08 mL of bacteriostatic water to the vial containing 5 mg of powder to achieve a concentration of 2.4 mg/mL.

Doses of Semaglutide

Here we provide an overview of methods for reconstituting 2 mg and 5 mg vials to achieve the semaglutide dosages for long-term weight management.

Semaglutide Reconstitution 2mg vial

Dose	0.25 mg	0.5 mg	1 mg	1.7 mg
Volume of bacteriostatic water (mL)*	2 mL	2 mL	1 mL	1 mL
Volume of solution in the syringe (mL)**	0.25 mL	0.5 mL	0.5 mL	1 mL
Number of doses in one vial***	8	4	2	1
Concentration per 1 mL	1 mg/mL	1 mg/mL	2 mg/mL	2 mg/mL

*This column tells you how much bacteriostatic water you should add to the vial.
**This column tells you how much of the produced solution you should draw up in the syringe so that the syringe contains the desired dose.
***This column tells how many times you can draw up the desired dose with the syringe per vial.

Semaglutide Reconstitution 5mg vial

Dose	0.5 mg	1 mg	1.7 mg	2.4 mg
Volume of bacteriostatic water (mL)*	2 mL	1 mL	1 mL	1 mL
Volume of solution in the syringe (mL)**	0.2 mL	0.2 mL	0.33 mL	0.5 mL
Number of doses in one vial***	10	5	3	2
Concentration per 1 mL	5 mg/mL	2.5 mg/mL	2.5 mg/mL	2.5 mg/mL

Semaglutide Mixing Instructions

Remove the plastic cap to expose the rubber stopper on the semaglutide vial.
Do the same for the bacteriostatic water vial, removing its plastic cap to reveal the rubber stopper.
Use alcohol pads to clean the rubber stoppers on both vials to prevent bacterial contamination.
Pierce the rubber stopper of the bacteriostatic water vial with the syringe needle.
Draw the required amount of bacteriostatic water by pulling the syringe’s plunger back to the appropriate measurement mark.
After filling the syringe with bacteriostatic water, withdraw the needle from this vial and insert it into the semaglutide vial’s rubber stopper.
Inject the bacteriostatic water into the semaglutide vial slowly.
Gently swirl the vial to ensure the peptide dissolves completely, being careful to avoid shaking the vial.

How to Store Semaglutide After Reconstitution

Temperature

Store reconstituted Semaglutide at controlled temperature, not exceeding 8°C (46,4°F).

Labeling

Clearly label the vial with the date of dilution to track expiration and ensure timely use.

Aliquoting

Consider aliquoting a reconstituted solution to avoid repeated punctures while maintaining sterility and potency.

Avoid Freeze-Thaw Cycles

To avoid damage, refrain from repeatedly freezing and thawing reconstituted Semaglutide.

Learn More About Semaglutide

If you have any questions about Semaglutide, explore our FAQ section for detailed answers.